API (Analytical Profile Index) 20E presented is a biochemical panel for identification and differentiation of members of the family. Changes to thesaurus. Changes to database old new. Taxons. Notes. Identification. Additional tests. API® 20 E v v X. X. X. X. RapiD 20 E™ v v X. API® 20 Microbial Identification Kits, bioMerieux. Supplier: Quantity. API® 20 E Microbial Identification Kit, , , , Pack of 25, Retrieving.
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Yellow-green is indicative of a negative result. Record the results under the appropriate heading on the report form.
Qualified Personnel should collect specimens according to standards routinely required for specimen handling The following chart gives the expected results on the Microbact System after an hour incubation: Microbact Biochemical Identification Kits. Gram-stain, colonial morphology, source of isolate, percent probability degree of separationtests against, additional test indications and results, frequency of ID choice and antibioGram. Argine dihydrolase converts arginine into ornithine, ammonia and carbon dioxide.
Using a sterile pipette or dropper bottle, overlay the substrates underlined on the holding tray with sterile mineral oil, i. Evaluate 15 to 30 minutes after the addition of reagents. When Actinobacillus or Pasteurella spp.
API PANEL READING
Used materials should be autoclaved, incinerated, or immersed in germicide before disposal. When Actinobacillus or Pasteurella sp. After the addition of Nitrate reagents A and B: Incubation Reseal the inoculated rows with the adhesive seal and write the specimen identification number on the end 200e with a marker pen.
A small amount of zinc powder should be added to those wells which exhibit a yellow colour after the addition of galedie nitrate reagents.
Indole is formed from metabolism of tryptophan. Preparation of inoculum Pick isolated colonies from an hour culture and emulsify in 2. The results should be interpreted as follows:. Set up proceedure Isolation An hour pure culture of the gwlerie to be identified must be obtained.
API for Microorganism Identification | bioMérieux
To determine the purity of the inoculum, it is advisable to inoculate a solid non-selective medium with the test suspension to act as a culture purity check. This causes a pH rise as indicated by bromothymol blue. MBA Microbact 12E 20 pouches, identifications. Refer to the procedure chart for a condensed version of the following procedures. Test can be evaluated immediately after the addition of the reagent.
The 12B strips can be used alongside the 12E, but in a separate tray. The reactions are evaluated as positive or negative by comparing them with the colour chart. Acetoin is produced from glucose indicated by the formation of a pink-red complex after the addition of alpha-naphthol and creatine.
MB, Microbact GNB 12A () | Oxoid – Product Detail
All organisms belonging to the family Enterobacteriaceae reduce nitrates to nitrites and give a positive reaction. Quality control The overall performance of the system should be monitored by testing appropriate control strains.
Using a sterile Pasteur pipette, transfer one drop of the peptone water culture into the appropriate volume see Procedure Chart of sterile saline solution 0.
Bromothymol blue indicator changes from blue to yellow when the carbohydrate is fermented. The following halerie are recommended for independent laboratory assessment.
Well 8 for 12B and 20 for 24E is not overlayed with oil for oxidase- positive, miscellaneous Gram-negative bacilli. Reading the test strip 1. Well 1 lysine Well 2 ornithine Well 3 H 2 S. The 12 substrates contained in the 12A strips are available in a solid microplate format, galeri to as 12E.
The lysine and ornithine decarboxylase reactions should be carefully interpreted. Bromothymol blue is the indicator. Indole Kovacs reagent forms a pink-red complex with indole. All systems should be read after 48 hours for the identification of Miscellaneous Gram-negative bacilli. Reactions obtained using the Microbact System may differ from published results using other substrate formulations.
Bromothymol blue indicates formation of the specific amine cadaverine.
Indole positive organisms may produce a brown colour. These can be purchased individually or as a set by ordering product code MB Inoculation The wells of individual substrate sets can be exposed by cutting the end tag of the sealing strip and slowly peeling it back.
One drop of Nitrate reagent A and 1 galere of Nitrate reagent B is added to the well.
In addition, motility and nitrate test should be performed for miscellaneous Gram-negative bacilli. This will determine whether nitrate has been reduced completely to nitrogen gas N2.
An organism unable to utilize this substrate results in the accumulation of succinic acid and the organism cannot grow. Galeri Aspects and Perspectives.